Singleton, the first African-American man to be nominated for a Best Directing Oscar, died on April 29. He was 51.
His family announced that day they were taking the director off of life support 13 days after he suffered a stroke.
"It is with heavy hearts we announce that our beloved son, father and friend, John Daniel Singleton will be taken off of life support today. This was an agonizing decision, one that our family made, over a number of days, with the careful counsel of John's doctors," the statement began.
Singleton suffered a "major stroke" after arriving back in the U.S. from a trip to Costa Rica. His mother, Shelia Ward, filed an application to establish a temporary conservatorship for his estate in April.